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Palmitoylethanolamide ameliorates hippocampal damage and behavioral dysfunction after perinatal asphyxia in the immature rat brain

dc.contributor.authorHerrera M.I.
dc.contributor.authorUdovin L.D.
dc.contributor.authorToro-Urrego N.
dc.contributor.authorKusnier C.F.
dc.contributor.authorLuaces J.P.
dc.contributor.authorCapani F.
dc.date.accessioned2020-09-02T22:20:27Z
dc.date.available2020-09-02T22:20:27Z
dc.date.issued2018
dc.identifier10.3389/fnins.2018.00145
dc.identifier.citation12, MAR, -
dc.identifier.issn16624548
dc.identifier.urihttps://hdl.handle.net/20.500.12728/4876
dc.descriptionPerinatal asphyxia (PA) is an obstetric complication associated with an impaired gas exchange. This health problem continues to be a determinant of neonatal mortality and neurodevelopmental disorders. Palmitoylethanolamide (PEA) has exerted neuroprotection in several models of brain injury and neurodegeneration. We aimed at evaluating the potential neuroprotective role of PEA in an experimental model, which induces PA in the immature rat brain. PA was induced by placing Sprague Dawley newborn rats in a water bath at 37°C for 19 min. Once their physiological conditions improved, they were given to surrogate mothers that had delivered normally within the last 24 h. The control group was represented by non-fostered vaginally delivered pups, mimicking the clinical situation. Treatment with PEA (10 mg/kg) was administered within the first hour of life. Modifications in the hippocampus were analyzed with conventional electron microscopy, immunohistochemistry (for NeuN, pNF-H/M, MAP-2, and GFAP) and western blot (for pNF H/M, MAP-2, and GFAP). Behavior was also studied throughout Open Field (OF) Test, Passive Avoidance (PA) Task and Elevated Plus Maze (EPM) Test. After 1 month of the PA insult, we observed neuronal nucleus degeneration in CA1 using electron microscopy. Immunohistochemistry revealed a significant increase in pNF-H/M and decrease in MAP-2 in CA1 reactive area. These changes were also observed when analyzing the level of expression of these markers by western blot. Vertical exploration impairments and anxiety-related behaviors were encountered in the OF and EPM tests. PEA treatment attenuated PA-induced hippocampal damage and its corresponding behavioral alterations. These results contribute to the elucidation of PEA neuroprotective role after PA and the future establishment of therapeutic strategies for the developing brain. © 2018 Herrera, Udovin, Toro-Urrego, Kusnier, Luaces and Capani.
dc.language.isoen
dc.publisherFrontiers Media S.A.
dc.subjectCA1 region
dc.subjectHippocampus
dc.subjectNeuroprotection
dc.subjectPalmitoylethanolamide
dc.subjectPerinatal asphyxia
dc.subjectglial fibrillary acidic protein
dc.subjectmicrotubule associated protein 2
dc.subjectneurofilament H protein
dc.subjectneurofilament M protein
dc.subjectneurofilament protein
dc.subjectneuron specific nuclear protein
dc.subjectpalmidrol
dc.subjectunclassified drug
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectanxiety
dc.subjectArticle
dc.subjectbehavior disorder
dc.subjectbrain damage
dc.subjectbrain hypoxia
dc.subjectcontrolled study
dc.subjectdrug effect
dc.subjectelevated plus maze test
dc.subjectexploratory behavior
dc.subjectfemale
dc.subjecthippocampal CA1 region
dc.subjectmale
dc.subjectnerve cell degeneration
dc.subjectneuroprotection
dc.subjectnewborn
dc.subjectnonhuman
dc.subjectopen field test
dc.subjectpassive avoidance test
dc.subjectperinatal asphyxia
dc.subjectprotein expression
dc.subjectrat
dc.titlePalmitoylethanolamide ameliorates hippocampal damage and behavioral dysfunction after perinatal asphyxia in the immature rat brain
dc.typeArticle


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