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dc.contributor.authorHernández-Moreno D.
dc.contributor.authorDe La Casa-Resino I.
dc.contributor.authorFlores J.M.
dc.contributor.authorGonzález-Gómez M.J.
dc.contributor.authorNeila C.M.
dc.contributor.authorSoler F.
dc.contributor.authorPérez-López M.
dc.date.accessioned2020-09-02T22:20:03Z
dc.date.available2020-09-02T22:20:03Z
dc.date.issued2014
dc.identifier10.2478/10004-1254-65-2014-2538
dc.identifier.citation65, 3, 311-318
dc.identifier.issn00041254
dc.identifier.urihttps://hdl.handle.net/20.500.12728/4855
dc.descriptionThis study investigated the influence of the pesticide methomyl on different enzymatic activities in carp. The fish were exposed to a sub-lethal concentration (0.34 mg L-1) of methomyl for 15 days. On days 4 and 15, catalase (CAT) and glutathione-S-transferase (GST) activities were measured in the liver and gills. Acetylcholinesterase (AChE) activity in brain and muscle was also determined. Liver catalase activity slightly increased in exposed fish when compared to controls, but it was statistically significant only at the beginning of the experiment. No changes in CAT activity in the gills of exposed and control animals were observed (mean values were in the range 10.7-11.7 nmol min-1 per mg of protein). Liver GST activity was slightly inhibited in the exposed animals at the beginning of the study; however, it was significantly inhibited in the gills. Brain AChE activity was diminished throughout the experiment and significantly decreased after 96 h of exposure compared to controls (0.041 vs. 0.075 nmol min-1 per mg of protein; p<0.001). Our findings suggest that CAT, GST, and AChE are reliable biomarkers of effect after exposure to methomyl.
dc.language.isoen
dc.publisherInstitute for Medical Research and Occupational Health
dc.subjectAcetylcholinesterase
dc.subjectCarbamate
dc.subjectCatalase
dc.subjectFish
dc.subjectGlutathione S-transferase
dc.subjectacetylcholinesterase
dc.subjectbiological marker
dc.subjectcarbamic acid derivative
dc.subjectcatalase
dc.subjectglutathione transferase
dc.subjectmethomyl
dc.subjectpyrethroid
dc.subjectacetylcholinesterase
dc.subjectbiological marker
dc.subjectcatalase
dc.subjectglutathione transferase
dc.subjectinsecticide
dc.subjectmethomyl
dc.subjectwater pollutant
dc.subjectanimal tissue
dc.subjectArticle
dc.subjectbrain
dc.subjectcarp
dc.subjectcontrolled study
dc.subjectenvironmental change
dc.subjectenvironmental monitoring
dc.subjectenzyme activity
dc.subjectexposure
dc.subjectgill
dc.subjecthigh performance liquid chromatography
dc.subjectliver
dc.subjectmuscle
dc.subjectnonhuman
dc.subjectanimal
dc.subjectcarp
dc.subjectdrug effects
dc.subjectenvironmental exposure
dc.subjectmetabolism
dc.subjectoxidative stress
dc.subjecttoxicity
dc.subjectwater pollutant
dc.subjectAnimalia
dc.subjectCyprinidae
dc.subjectCyprinus carpio
dc.subjectAcetylcholinesterase
dc.subjectAnimals
dc.subjectBiomarkers
dc.subjectBrain
dc.subjectCarps
dc.subjectCatalase
dc.subjectEnvironmental Exposure
dc.subjectGills
dc.subjectGlutathione Transferase
dc.subjectInsecticides
dc.subjectLiver
dc.subjectMethomyl
dc.subjectMuscles
dc.subjectOxidative Stress
dc.subjectWater Pollutants, Chemical
dc.titleDifferent enzymatic activities in carp (Cyprinus carpio L.) as potential biomarkers of exposure to the pesticide methomyl
dc.typeArticle


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