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Equol status and changes in fecal microbiota in menopausal women receiving long-term treatment for menopause symptoms with a soy-isoflavone concentrate

dc.contributor.authorGuadamuro L.
dc.contributor.authorDelgado S.
dc.contributor.authorRedruello B.
dc.contributor.authorFlórez A.B.
dc.contributor.authorSuárez A.
dc.contributor.authorMartínez-Camblor P.
dc.contributor.authorMayo B.
dc.date.accessioned2020-09-02T22:19:29Z
dc.date.available2020-09-02T22:19:29Z
dc.date.issued2015
dc.identifier10.3389/fmicb.2015.00777
dc.identifier.citation6, AUG, -
dc.identifier.issn1664302X
dc.identifier.urihttps://hdl.handle.net/20.500.12728/4755
dc.descriptionThe knowledge regarding the intestinal microbial types involved in isoflavone bioavailability and metabolism is still limited. The present work reports the influence of a treatment with isoflavones for 6 months on the fecal bacterial communities of 16 menopausal women, as determined by culturing and culture-independent microbial techniques. Changes in fecal communities were analyzed with respect to the women's equol-producing phenotype. Compared to baseline, at 1 and 3 months the counts for all microbial populations in the feces of equol-producing women had increased strongly. In contrast, among the non-producers, the counts for all microbial populations at 1 month were similar to those at baseline, and decreased significantly by 3 and 6 months. Following isoflavone intake, major bands in the denaturing gradient gel electrophoresis (DGGE) profiles appeared and disappeared, suggesting important changes in majority populations. In some women, increases were seen in the intensity of specific DGGE bands corresponding to microorganisms known to be involved in the metabolism of dietary phytoestrogens (Lactonifactor longoviformis, Faecalibacterium prausnitzii, Bifidobacterium sp., Ruminococcus sp.). Real-Time quantitative PCR revealed that the Clostridium leptum and C. coccoides populations increased in equol producers, while those of bifidobacteria and enterobacteria decreased, and vice versa in the non-producers. Finally, the Atopobium population increased in both groups, but especially in the non-producers at three months. As the main findings of this study, (i) variations in the microbial communities over the 6-month period of isoflavone supplementation were large; (ii) no changes in the fecal microbial populations that were convincingly treatment-specific were seen; and (iii) the production of equol did not appear to be associated with the presence of, or increase in the population of, any of the majority bacterial types analyzed. © 2015 Guadamuro, Delgado, Redruello, Flórez, Suárez, Martínez-Camblor and Mayo.
dc.language.isoen
dc.publisherFrontiers Media S.A.
dc.subjectEquol
dc.subjectFecal microbiota
dc.subjectIntestinal microbiology
dc.subjectMenopause
dc.subjectProbiotics
dc.subjectSoy isoflavone
dc.subjectcreatinine
dc.subjectequol
dc.subjectisoflavone derivative
dc.subjectphytoestrogen
dc.subjectsoy isoflavone concentrate
dc.subjectunclassified drug
dc.subjectadult
dc.subjectArticle
dc.subjectbacterial count
dc.subjectBifidobacterium
dc.subjectClostridium
dc.subjectcomparative study
dc.subjectculture technique
dc.subjectdenaturing gradient gel electrophoresis
dc.subjectdiet supplementation
dc.subjectdrug effect
dc.subjectFaecalibacterium prausnitzii
dc.subjectfeces analysis
dc.subjectfeces microflora
dc.subjectfemale
dc.subjectgene amplification
dc.subjectgene sequence
dc.subjecthuman
dc.subjectmenopausal syndrome
dc.subjectmiddle aged
dc.subjectnonhuman
dc.subjectreal time polymerase chain reaction
dc.subjectRuminococcus
dc.subjectultra performance liquid chromatography
dc.subjecturinalysis
dc.titleEquol status and changes in fecal microbiota in menopausal women receiving long-term treatment for menopause symptoms with a soy-isoflavone concentrate
dc.typeArticle


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