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dc.contributor.authorGarcía-Mesa Y.
dc.contributor.authorGarcía-Piqueras J.
dc.contributor.authorGarcía B.
dc.contributor.authorFeito J.
dc.contributor.authorCabo R.
dc.contributor.authorCobo J.
dc.contributor.authorVega J.A.
dc.contributor.authorGarcía-Suárez O.
dc.date.accessioned2020-09-02T22:18:50Z
dc.date.available2020-09-02T22:18:50Z
dc.date.issued2017
dc.identifier10.1111/joa.12688
dc.identifier.citation231, 6, 978-989
dc.identifier.issn00218782
dc.identifier.urihttps://hdl.handle.net/20.500.12728/4638
dc.descriptionThe transformation of mechanical energy into electrical signals is the first step in mechanotransduction in the peripheral sensory nervous system and relies on the presence of mechanically gated ion channels within specialized sensory organs called mechanoreceptors. Piezo2 is a vertebrate stretch-gated ion channel necessary for mechanosensitive channels in mammalian cells. Functionally, it is related to light touch, which has been detected in murine cutaneous Merkel cell–neurite complexes, Meissner-like corpuscles and lanceolate nerve endings. To the best of our knowledge, the occurrence of Piezo2 in human cutaneous mechanoreceptors has never been investigated. Here, we used simple and double immunohistochemistry to investigate the occurrence of Piezo2 in human digital glabrous skin. Piezo2 immunoreactivity was detected in approximately 80% of morphologically and immunohistochemically characterized (cytokeratin 20+, chromogranin A+ and synaptophisin+) Merkel cells. Most of them were in close contact with Piezo2− nerve fibre profiles. Moreover, the axon, but not the lamellar cells, of Meissner's corpuscles was also Piezo2+, but other mechanoreceptors, i.e. Pacinian or Ruffini's corpuscles, were devoid of immunoreactivity. Piezo2 was also observed in non-nervous tissue, especially the basal keratinocytes, endothelial cells and sweat glands. The present results demonstrate the occurrence of Piezo2 in cutaneous sensory nerve formations that functionally work as slowly adapting (Merkel cells) and rapidly adapting (Meissner's corpuscles) low-threshold mechanoreceptors and are related to fine and discriminative touch but not to vibration or hard touch. These data offer additional insight into the molecular basis of mechanosensing in humans. © 2017 Anatomical Society
dc.language.isoen
dc.publisherBlackwell Publishing Ltd
dc.subjectcutaneous mechanoreceptors
dc.subjectmechanotransduction
dc.subjectMeissner's corpuscles
dc.subjectMerkel cells
dc.subjectPiezo2 ion channel
dc.subjectchromogranin A
dc.subjectcytokeratin 20
dc.subjectmembrane protein
dc.subjectPiezo2 protein
dc.subjectunclassified drug
dc.subjection channel
dc.subjectPIEZO2 protein, human
dc.subjectadult
dc.subjectArticle
dc.subjectaxon
dc.subjectcontrolled study
dc.subjectfemale
dc.subjecthuman
dc.subjecthuman cell
dc.subjecthuman tissue
dc.subjectimmunohistochemistry
dc.subjectimmunoreactivity
dc.subjectkeratinocyte
dc.subjectlamellar body
dc.subjectmale
dc.subjectmechanotransduction
dc.subjectMerkel cell
dc.subjectmorphotype
dc.subjectpriority journal
dc.subjectprotein expression
dc.subjectprotein localization
dc.subjectquantitative study
dc.subjectskin nerve
dc.subjectskin receptor
dc.subjectsubmucous plexus
dc.subjectbiosynthesis
dc.subjectfinger
dc.subjectinnervation
dc.subjectmechanoreceptor
dc.subjectMerkel cell
dc.subjectmetabolism
dc.subjectmiddle aged
dc.subjectphysiology
dc.subjectskin
dc.subjectyoung adult
dc.subjectAdult
dc.subjectFemale
dc.subjectFingers
dc.subjectHumans
dc.subjectIon Channels
dc.subjectMale
dc.subjectMechanoreceptors
dc.subjectMechanotransduction, Cellular
dc.subjectMerkel Cells
dc.subjectMiddle Aged
dc.subjectSkin
dc.subjectYoung Adult
dc.titleMerkel cells and Meissner's corpuscles in human digital skin display Piezo2 immunoreactivity
dc.typeArticle


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