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Nestin+cells forming spheroids aggregates resembling tumorspheres in experimental ENU-induced gliomas

dc.contributor.authorGarcía-Blanco A.
dc.contributor.authorBulnes S.
dc.contributor.authorPomposo I.
dc.contributor.authorCarrasco A.
dc.contributor.authorLafuente J.V.
dc.date.accessioned2020-09-02T22:18:46Z
dc.date.available2020-09-02T22:18:46Z
dc.date.issued2016
dc.identifier10.14670/HH-11-763
dc.identifier.citation31, 12, 1347-1356
dc.identifier.issn02133911
dc.identifier.urihttps://hdl.handle.net/20.500.12728/4616
dc.descriptionNestin+cells from spheroid aggregates display typical histopathological features compatible with cell stemness. Nestin and CD133+cells found in glioblastomas, distributed frequently around aberrant vessels, are considered as potential cancer stem cells. They are possible targets for antitumoral therapy because they lead the tumorigenesis, invasiveness and angiogenesis. However, little is known about their role and presence in low-grade gliomas. The aim of this work is to localize and characterize the distribution of these cells inside tumors during the development of experimental endogenous glioma. For this study, a single dose of Ethyl-nitrosourea was injected into pregnant rats. Double immunofluorescences were performed in order to identify stem-like and differentiated cells. Low-grade gliomas display Nestin+cells distributed throughout the tumor. More malignant gliomas show, in addition to that, a perivascular location with some Nestin+cells co-expressing CD133 or VEGF, and the intratumoral spheroid aggregates of Nestin/CD133+ cells. These structures are encapsulated by welldifferentiated VEGF/GFAP+cells. Spheroid aggregates increase in size in the most malignant stages. Spheroid aggregates have morphological and phenotypic similarities to in vitro neurospheres and could be an in vivo analogue of them. These arrangements could be a reservoir of undifferentiated cells formed to escape adverse microenvironments. © 2016, Histology and Histopathology. All rights reserved.
dc.language.isoen
dc.publisherHistology and Histopathology
dc.subjectENU
dc.subjectGlioma
dc.subjectNestin
dc.subjectStem cells
dc.subjectcarcinogen
dc.subjectethylnitrosourea
dc.subjectNes protein, rat
dc.subjectnestin
dc.subjecttumor marker
dc.subjectanimal
dc.subjectbiosynthesis
dc.subjectbrain tumor
dc.subjectcancer stem cell
dc.subjectglioma
dc.subjectimmunohistochemistry
dc.subjectpathology
dc.subjectrat
dc.subjectSprague Dawley rat
dc.subjectAnimals
dc.subjectBiomarkers, Tumor
dc.subjectBrain Neoplasms
dc.subjectCarcinogens
dc.subjectEthylnitrosourea
dc.subjectGlioma
dc.subjectImmunohistochemistry
dc.subjectNeoplastic Stem Cells
dc.subjectNestin
dc.subjectRats
dc.subjectRats, Sprague-Dawley
dc.titleNestin+cells forming spheroids aggregates resembling tumorspheres in experimental ENU-induced gliomas
dc.typeArticle


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