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Gene expression of Vibrio parahaemolyticus growing in laboratory isolation conditions compared to those common in its natural ocean environment

dc.contributor.authorGarcía K.
dc.contributor.authorYáñez C.
dc.contributor.authorPlaza N.
dc.contributor.authorPeña F.
dc.contributor.authorSepúlveda P.
dc.contributor.authorPérez-Reytor D.
dc.contributor.authorEspejo R.T.
dc.date.accessioned2020-09-02T22:18:45Z
dc.date.available2020-09-02T22:18:45Z
dc.date.issued2017
dc.identifier10.1186/s12866-017-1030-6
dc.identifier.citation17, 1, -
dc.identifier.issn14712180
dc.identifier.urihttps://hdl.handle.net/20.500.12728/4609
dc.descriptionBackground: Vibrio parahaemolyticus is an autochthonous marine bacterial species comprising strains able to grow in broth containing bile salts at 37 °C, a condition seldom found in the ocean. However, this condition is used for isolation in the laboratory because it is considered a necessary property for pathogenesis. In this context, revealing how gene expression enables V. parahaemolyticus to adapt to this particular condition -common to almost all V. parahaemolyticus isolates- will improve our understanding of the biology of this important pathogen. To determine the genes of V. parahaemolyticus differentially expressed when growing in isolation condition (37 °C, 0.9% NaCl, and 0.04% bile salts) referred to those at the temperature and salt concentration prevailing in ocean south of Chile (marine-like condition; 12 °C, 3% NaCl, and absence of bile salts) we used high-throughput sequencing of RNA. Results: Our results showed that in the isolation condition, among the 5034 genes annotated in the V. parahaemolyticus RIMD2210633 genome, 344 were upregulated and 433 downregulated referred to the marine-like condition, managing an adjusted P-value (Padj) < E−5. Between the 50 more highly expressed genes, among the small RNAs (sRNA), the three carbon storage regulators B (CsrB) were up four to six times, while RyhB, related to iron metabolism besides motility control, was down about eight times. Among proteins, BfdA, a hemolysin-co-regulated protein (Hcp1) secreted by T6SS1, one of the most highly expressed genes, was about 140 times downregulated in isolation condition. The highest changes in relative expression were found among neighboring genes coding for proteins related to respiration, which were about 40 times upregulated. Conclusions: When V. parahaemolyticus is grown in conditions used for laboratory isolation 777 genes are up- or downregulated referred to conditions prevailing in the marine-like condition; the most significantly overrepresented categories among upregulated processes were those related to transport and localization, while secretion and pathogenesis were overrepresented among downregulated genes. Genes with the highest differential expression included the sRNAs CsrB and RhyB and the mRNAs related with secretion, nutritional upshift, respiration and rapid growing. © 2017 The Author(s).
dc.language.isoen
dc.publisherBioMed Central Ltd.
dc.subjectBioinformatics
dc.subjectGenome
dc.subjectmRNA
dc.subjectRNA seq
dc.subjectsRNA
dc.subjectVibrio parahaemolyticus
dc.subjectArticle
dc.subjectbacterial gene
dc.subjectbacterial strain
dc.subjectbacterium isolation
dc.subjectBfdA gene
dc.subjectcarbon storage
dc.subjectcontrolled study
dc.subjectCsrB gene
dc.subjectdown regulation
dc.subjectgene expression
dc.subjectgene location
dc.subjectgene sequence
dc.subjectHcp1 gene
dc.subjectiron metabolism
dc.subjectnonhuman
dc.subjectRyhB gene
dc.subjectupregulation
dc.subjectVibrio parahaemolyticus
dc.subjectchemistry
dc.subjectChile
dc.subjectculture medium
dc.subjectenvironment
dc.subjectgene expression regulation
dc.subjectgenetics
dc.subjectgrowth, development and aging
dc.subjecthigh throughput screening
dc.subjectisolation and purification
dc.subjectmicrobiology
dc.subjectnucleotide sequence
dc.subjectpathogenicity
dc.subjectphysiology
dc.subjectsea
dc.subjectsecretion (process)
dc.subjectsequence alignment
dc.subjecttemperature
dc.subjectVibrio parahaemolyticus
dc.subjectvibriosis
dc.subjectbacterial protein
dc.subjectbacterial RNA
dc.subjectsea water
dc.subjectBacterial Proteins
dc.subjectBase Sequence
dc.subjectChile
dc.subjectCulture Media
dc.subjectEnvironment
dc.subjectGene Expression Regulation, Bacterial
dc.subjectGenes, Bacterial
dc.subjectHigh-Throughput Screening Assays
dc.subjectOceans and Seas
dc.subjectRNA, Bacterial
dc.subjectSeawater
dc.subjectSequence Alignment
dc.subjectTemperature
dc.subjectVibrio Infections
dc.subjectVibrio parahaemolyticus
dc.titleGene expression of Vibrio parahaemolyticus growing in laboratory isolation conditions compared to those common in its natural ocean environment
dc.typeArticle


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