Sex determination by observation of barr body in teeth subjected to high temperatures [Determinación del sexo mediante la observación de la cromatina de barr en piezas sometidas a altas temperatures]

Galdames I.S.; Flores A.; Roa I.; Cantín M.; Zavando D.

dc.contributor.author	Galdames I.S.
dc.contributor.author	Flores A.
dc.contributor.author	Roa I.
dc.contributor.author	Cantín M.
dc.contributor.author	Zavando D.
dc.date.accessioned	2020-09-02T22:18:20Z
dc.date.available	2020-09-02T22:18:20Z
dc.date.issued	2011
dc.identifier	10.4067/s0717-95022011000100034
dc.identifier.citation	29, 1, 199-203
dc.identifier.issn	07179367
dc.identifier.uri	https://hdl.handle.net/20.500.12728/4566
dc.description	Sex determination is one of the keys in the identification process. A useful histological method for sex determination is the observation of Barr chromatin or Barr body. This study determines the effect of high temperatures on the diagnostic performance of the Barr chromatin observation on teeth. Were used 50 healthy teeth from 25 male and 25 female individuals aged between 14 and 44 years. The teeth were divided into 5 groups (each group with 5 female and 5 male teeth) and were exposed to controlled temperatures of 200, 400, 600, 800, and 1000 degrees C for 5 minutes. The coronal pulp was obtained and the tissue was processed and stained with hematoxylin-eosin. Four histological slides of male and 4 of female individuals were randomly selected, for each temperature level, which were observed by conventional microscopy at 100X magnification, each showing 50 cells per plate. The presence of 1 cell with visible sex chromatin was considered positive for females. It was only possible to evaluate the samples from groups subjected to 200 and 400 degrees C. In the groups analyzed, the test showed 100% accuracy. The average number of cells found to be positive Barr chromatin was 15 (SD 3.9) at 200 degrees C and 11 (SD 2.8) at 400 degrees C. Hence, it was possible to detect the sex at these temperatures by observing chromatin of the Barr body in dental pulp.
dc.language.iso	en
dc.language.iso	es
dc.publisher	Universidad de la Frontera
dc.subject	Barr chromatin
dc.subject	Forensic dentistry
dc.subject	Sex determination
dc.title	Sex determination by observation of barr body in teeth subjected to high temperatures [Determinación del sexo mediante la observación de la cromatina de barr en piezas sometidas a altas temperatures]
dc.type	Article

Ficheros en el ítem

Nombre:: item_2-s2.0-79957449648.pdf
Tamaño:: 2.516Kb
Formato:: PDF

Ver/

Este ítem aparece en la(s) siguiente(s) colección(ones)

Artículos científicos

Mostrar el registro sencillo del ítem

Repositorio Académico

Sex determination by observation of barr body in teeth subjected to high temperatures [Determinación del sexo mediante la observación de la cromatina de barr en piezas sometidas a altas temperatures]

Ficheros en el ítem

Este ítem aparece en la(s) siguiente(s) colección(ones)

Ítems relacionados

Determination of the species from skeletal remains through histomorphometric evaluation and discriminant analysis [Determinación de la Especie a Partir de Restos Óseos Mediante Evaluación Histomorfométrica y Análisis Discriminante] (2020) ﻿

Observation of sex chromatin in exhumed bones, evaluation of their diagnosis value [Observación de la cromatina sexual en osamentas exhumadas, evaluación de su valor diagnóstico] (2020) ﻿

Evaluation of the baudoin condylar index diagnostic test for sex determination [Evaluación de la prueba diagnóstica índice condíleo de baudoin para determinación del sexo] (2020) ﻿

Determination of the species from skeletal remains through histomorphometric evaluation and discriminant analysis [Determinación de la Especie a Partir de Restos Óseos Mediante Evaluación Histomorfométrica y Análisis Discriminante] (2020)

Observation of sex chromatin in exhumed bones, evaluation of their diagnosis value [Observación de la cromatina sexual en osamentas exhumadas, evaluación de su valor diagnóstico] (2020)

Evaluation of the baudoin condylar index diagnostic test for sex determination [Evaluación de la prueba diagnóstica índice condíleo de baudoin para determinación del sexo] (2020)