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Mucin 5B, carbonic anhydrase 9 and claudin 18 are potential theranostic markers of gallbladder carcinoma

dc.contributor.authorEspinoza J.A.
dc.contributor.authorRiquelme I.
dc.contributor.authorSagredo E.A.
dc.contributor.authorRosa L.
dc.contributor.authorGarcía P.
dc.contributor.authorBizama C.
dc.contributor.authorApud-Bell M.
dc.contributor.authorLeal P.
dc.contributor.authorWeber H.
dc.contributor.authorBenavente F.
dc.contributor.authorVargas S.
dc.contributor.authorRomero D.
dc.contributor.authorKalergis A.M.
dc.contributor.authorRoa J.C.
dc.date.accessioned2020-09-02T22:17:19Z
dc.date.available2020-09-02T22:17:19Z
dc.date.issued2019
dc.identifier10.1111/his.13797
dc.identifier.citation74, 4, 597-607
dc.identifier.issn03090167
dc.identifier.urihttps://hdl.handle.net/20.500.12728/4394
dc.descriptionAims: Gallbladder cancer (GBC) is an aggressive tumour that is usually diagnosed at advanced stages and is characterised by a poor prognosis. Using public data of normal human tissues, we found that mRNA and protein levels of mucin 5B (MUC5B) and carbonic anhydrase 9 (CA9) were highly increased in gallbladder tissues. In addition, previous evidence has shown that claudin 18 (CLDN18) protein expression is higher in GBC. The aim of this study was to perform an analysis of these cell surface proteins during the histological progression of GBC in order to identify their theranostic potential. Methods and results: MUC5B expression, CA9 expression and CLDN18 expression were examined by immunohistochemistry in a series of 179 chronic cholecystitis (including 16 metaplastic tissues), 15 dysplasia and 217 GBC samples by the use of tissue microarray analysis. A composite staining score was calculated from staining intensity and percentage of positive cells. Immunohistochemical analysis showed high expression of MUC5B and CA9 among normal epithelium, metaplastic tissues, and dysplastic tissues. However, expression of both proteins was observed in roughly 50% of GBC samples. In contrast, CLDN18 was absent in normal epithelium, but its expression was higher in metaplastic cells. Among GBC cases, approximately half showed high CLDN18 expression. No associations were found between MUC5B, CA9 and CLDN18 expression and any clinicopathological features. Conclusions: CLDN18 is a new metaplasia marker in gallbladder tissues, and is conserved in approximately half of GBC cases. MUC5B and CA9 are highly conserved during GBC histological progression. The three markers are potential theranostic markers, in particular CA9 and CLDN18, for which there are already targeted therapies available. © 2018 John Wiley & Sons Ltd
dc.language.isoen
dc.publisherBlackwell Publishing Ltd
dc.subjectbiomarker
dc.subjectCA9
dc.subjectCLDN18
dc.subjectGallbladder cancer
dc.subjectMUC5B
dc.subjecttheranostic
dc.subjectcarbonate dehydratase IX
dc.subjectclaudin 18
dc.subjectmessenger RNA
dc.subjectmucin 5B
dc.subjectprotein p53
dc.subjectproteome
dc.subjecttranscription factor Cdx2
dc.subjecttranscriptome
dc.subjecttumor marker
dc.subjectCA9 protein, human
dc.subjectcarbonate dehydratase IX
dc.subjectclaudin
dc.subjectCLDN18 protein, human
dc.subjectmucin 5B
dc.subjecttumor antigen
dc.subjecttumor marker
dc.subjectadult
dc.subjectadvanced cancer
dc.subjectaged
dc.subjectArticle
dc.subjectbile duct carcinoma
dc.subjectcancer growth
dc.subjectcancer patient
dc.subjectcarcinogenesis
dc.subjectchronic cholecystitis
dc.subjectclinical feature
dc.subjectcohort analysis
dc.subjectdysplasia
dc.subjectfemale
dc.subjectgallbladder carcinoma
dc.subjectgallbladder epithelium
dc.subjecthuman
dc.subjecthuman cell
dc.subjecthuman tissue
dc.subjectimmunohistochemistry
dc.subjectmajor clinical study
dc.subjectmale
dc.subjectmolecular pathology
dc.subjectmRNA expression level
dc.subjectpriority journal
dc.subjectprotein expression level
dc.subjecttissue microarray
dc.subjectbiosynthesis
dc.subjectgallbladder tumor
dc.subjectmiddle aged
dc.subjectpathology
dc.subjectprocedures
dc.subjecttheranostic nanomedicine
dc.subjectAdult
dc.subjectAged
dc.subjectAntigens, Neoplasm
dc.subjectBiomarkers, Tumor
dc.subjectCarbonic Anhydrase IX
dc.subjectClaudins
dc.subjectFemale
dc.subjectGallbladder Neoplasms
dc.subjectHumans
dc.subjectMale
dc.subjectMiddle Aged
dc.subjectMucin-5B
dc.subjectTheranostic Nanomedicine
dc.titleMucin 5B, carbonic anhydrase 9 and claudin 18 are potential theranostic markers of gallbladder carcinoma
dc.typeArticle


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