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Insight into the origin and evolution of the Vibrio parahaemolyticus pandemic strain

dc.contributor.authorEspejo R.T.
dc.contributor.authorGarcía K.
dc.contributor.authorPlaza N.
dc.date.accessioned2020-09-02T22:17:18Z
dc.date.available2020-09-02T22:17:18Z
dc.date.issued2017
dc.identifier10.3389/fmicb.2017.01397
dc.identifier.citation8, JUL, -
dc.identifier.issn1664302X
dc.identifier.urihttps://hdl.handle.net/20.500.12728/4390
dc.descriptionA strain of Vibrio parahaemolyticus that emerged in 1995 caused the first known pandemic involving this species. This strain comprises clonal autochthonous ocean-dwelling bacteria whose evolution has occurred in the ocean environment. The low sequence diversity in this population enabled the discovery of information on its origin and evolution that has been hidden in bacterial clones that have evolved over a long period. Multilocus sequencing and microarray analysis, together with phylogenetic analysis, of pandemic and pre-pandemic isolates has suggested that the founder clone was an O3:K6 non-pathogenic strain that initially acquired a toxRS/new region and subsequently acquired at least seven novel genomic islands. Sequencing and comparison of whole genomes later confirmed these early observations, and it confirmed that most of the genetic changes occurred via gene conversion involving horizontally transmitted DNA. The highly clonal population rapidly diversified, especially in terms of antigenicity, and 27 serotypes have already been reported. Comparisons of the core genomes derived from the founder clone indicate that there are only a few hundred single-nucleotide variations between isolates. However, when the whole genome is considered (the core plus non-core genome and from any clonal frame), the amount of DNA with a different clonal frame can reach up to 4.2% and the number of single-nucleotide variations can reach several hundred thousand. Altogether, these and previous observations based on multilocus sequence typing, microarray analysis, and whole-genome sequencing indicate the large contribution made by DNA with different clonal genealogy to genome diversification. The evidence also indicates that horizontal gene transfer (HGT) caused the emergence of new pathogens. Furthermore, the extent of HGT seems to depend on the vicissitudes of the life of each bacterium, as exemplified by differences in thousands of base pairs acquired by HGT among almost identical genetic isolates. © 2017 Espejo, García and Plaza.
dc.language.isoen
dc.publisherFrontiers Media S.A.
dc.subjectDiarrhea
dc.subjectEcology
dc.subjectGenomic
dc.subjectHorizontal gene transfer
dc.subjectPhylogeny
dc.subjectbacterial genetics
dc.subjectbioinformatics
dc.subjectcomparative genomic hybridization
dc.subjectdirect genome restriction enzyme analysis
dc.subjectevolutionary homology
dc.subjectgenealogy
dc.subjectgenetic isolation by distance
dc.subjectgenetic variability
dc.subjectgenomic island
dc.subjecthorizontal gene transfer
dc.subjectmicroarray analysis
dc.subjectmolecular biology
dc.subjectmultilocus sequence typing
dc.subjectnonhuman
dc.subjectphylogeny
dc.subjectpolymerase chain reaction
dc.subjectrestriction fragment length polymorphism
dc.subjectShort Survey
dc.subjectvariable number of tandem repeat
dc.subjectVibrio parahaemolyticus
dc.subjectVibrio parahaemolyticus O3:K6
dc.titleInsight into the origin and evolution of the Vibrio parahaemolyticus pandemic strain
dc.typeShort Survey


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