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Increased levels of the T-helper 22-associated cytokine (interleukin-22) and transcription factor (aryl hydrocarbon receptor) in patients with periodontitis are associated with osteoclast resorptive activity and severity of the disease

dc.contributor.authorDíaz-Zúñiga J.
dc.contributor.authorMelgar-Rodríguez S.
dc.contributor.authorRojas L.
dc.contributor.authorAlvarez C.
dc.contributor.authorMonasterio G.
dc.contributor.authorCarvajal P.
dc.contributor.authorVernal R.
dc.date.accessioned2020-09-02T22:16:53Z
dc.date.available2020-09-02T22:16:53Z
dc.date.issued2017
dc.identifier10.1111/jre.12461
dc.identifier.citation52, 5, 893-902
dc.identifier.issn00223484
dc.identifier.urihttps://hdl.handle.net/20.500.12728/4368
dc.descriptionBackground and Objective: Two new T-helper (Th) phenotypes have been recently described and named Th9 and Th22 lymphocytes; however, their role in the pathogenesis of periodontitis remains unclear. This study was aimed to assess whether Th9 and Th22 lymphocytes, through interleukin (IL)-9 and IL-22 production, respectively, are associated with the severity of periodontitis and bone resorption. Material and Methods: Gingival crevicular fluid samples and biopsies were obtained from patients with moderate-to-advanced chronic periodontitis and gingivitis, and healthy controls. The levels for the Th9 and Th22-associated cytokines and master-switch transcription factors Spi-B and aryl hydrocarbon receptor (AhR) were quantified by enzyme-linked immunosorbent assay, real-time reverse-transcription quantitative polymerase chain reaction and flow cytometry. In addition, the osteoclast activity in response to tissue homogenates from periodontitis and healthy samples was analyzed quantifying the number of TRAP-positive cells and areas of bone resorption pits produced, in the presence or absence of recombinant human IL-22 and anti-IL-22 neutralization antibody. Results: Higher levels of IL-22 and AhR were detected in patients with periodontitis compared with gingivitis and healthy individuals. In addition, higher levels of IL-9 and Spi-B were detected in gingivitis patients compared with periodontitis and healthy individuals. In patients with periodontitis, a significant positive correlation was detected between secreted levels of IL-22 and clinical attachment level of the sampled periodontal pockets. When osteoclasts were exposed to tissue homogenates obtained from patients with periodontitis, higher levels of resorptive activity were observed as compared with the same cells exposed to tissue homogenates obtained from healthy individuals, and this increment was dependent on the presence and neutralization of IL-22. Conclusion: Increased levels of IL-22 produced by Th22 lymphocytes are associated with the pathogenesis of periodontitis, in particular, with osteoclast resorptive activity and severity of disease. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
dc.language.isoen
dc.publisherBlackwell Munksgaard
dc.subjectAhR, transcription factor aryl hydrocarbon receptor
dc.subjectbone resorption
dc.subjectinterleukin-22
dc.subjectperiodontitis
dc.subjectTh22 lymphocytes
dc.subjectaromatic hydrocarbon receptor
dc.subjectcytokine
dc.subjectDNA binding protein
dc.subjectinterleukin 9
dc.subjectinterleukin derivative
dc.subjectinterleukin-22
dc.subjectRNA
dc.subjectRNA 18S
dc.subjectSPIB protein, human
dc.subjecttranscription factor
dc.subjectadult
dc.subjectchemistry
dc.subjectchronic periodontitis
dc.subjectfemale
dc.subjectgene expression
dc.subjectgenetics
dc.subjectgingivitis
dc.subjecthuman
dc.subjectimmunology
dc.subjectisolation and purification
dc.subjectmale
dc.subjectmetabolism
dc.subjectosteoclast
dc.subjectpathology
dc.subjectperiodontal disease
dc.subjectperiodontal pocket
dc.subjectsecretion (process)
dc.subjectAdult
dc.subjectChronic Periodontitis
dc.subjectCytokines
dc.subjectDNA-Binding Proteins
dc.subjectFemale
dc.subjectGene Expression
dc.subjectGingival Crevicular Fluid
dc.subjectGingivitis
dc.subjectHumans
dc.subjectInterleukin-9
dc.subjectInterleukins
dc.subjectMale
dc.subjectOsteoclasts
dc.subjectPeriodontal Attachment Loss
dc.subjectPeriodontal Pocket
dc.subjectReceptors, Aryl Hydrocarbon
dc.subjectRNA
dc.subjectRNA, Ribosomal, 18S
dc.subjectTranscription Factors
dc.titleIncreased levels of the T-helper 22-associated cytokine (interleukin-22) and transcription factor (aryl hydrocarbon receptor) in patients with periodontitis are associated with osteoclast resorptive activity and severity of the disease
dc.typeArticle


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