Mostrar el registro sencillo del ítem
Differential human Th22-lymphocyte response triggered by Aggregatibacter actinomycetemcomitans serotypes
dc.contributor.author | Díaz-Zúñiga J. | |
dc.contributor.author | Melgar-Rodríguez S. | |
dc.contributor.author | Monasterio G. | |
dc.contributor.author | Pujol M. | |
dc.contributor.author | Rojas L. | |
dc.contributor.author | Alvarez C. | |
dc.contributor.author | Carvajal P. | |
dc.contributor.author | Vernal R. | |
dc.date.accessioned | 2020-09-02T22:16:53Z | |
dc.date.available | 2020-09-02T22:16:53Z | |
dc.date.issued | 2017 | |
dc.identifier | 10.1016/j.archoralbio.2017.02.008 | |
dc.identifier.citation | 78, , 26-33 | |
dc.identifier.issn | 00039969 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12728/4367 | |
dc.description | Objective In Aggregatibacter actinomycetemcomitans, different serotypes have been described based on lipopolysaccharide (LPS) antigenicity. When T lymphocytes were stimulated with these serotypes, different patterns of T-helper (Th)1 and Th17-type of immune responses were reported. Recently, two new Th phenotypes have been described and named Th9 and Th22 lymphocytes; however, their role in the pathogenesis of periodontitis remains unclear. This study aimed to investigate the potential Th9 and/or Th22 lymphocyte responses when stimulated with autologous dendritic cells infected with different A. actinomycetemcomitans serotypes. Methods Monocyte-derived dendritic cells and naïve CD4+ T lymphocytes were obtained from healthy donors and stimulated with different serotypes of A. actinomycetemcomitans at a multiplicity of infection MOI = 102 or their purified LPS (10–50 ng/ml). The levels for the Th9 and Th22-associated cytokines, as well as the transcription factor master-switch genes implied in their differentiation Spi-B and AhR, were quantified by qPCR and ELISA. Results When stimulated with the serotype b of A. actinomycetemcomitans, higher levels of interleukin (IL)-6 and tumor necrosis factor (TNF)-α were detected in dendritic cells, as well as higher levels of IL-22 and AhR were detected in T lymphocytes, when compared with stimulation with the other serotypes. Conclusions The serotype b of A. actinomycetemcomitans has a higher capacity of trigger Th22-type of immune response in both dendritic cells and T lymphocytes. These data allow us to suggest that, when the serotype b of A. actinomycetemcomitans is a significant part of the subgingival biofilm, the Th22 polarization might be triggered within the periodontal lesion. © 2017 Elsevier Ltd | |
dc.language.iso | en | |
dc.publisher | Elsevier Ltd | |
dc.subject | Aggregatibacter actinomycetemcomitans | |
dc.subject | T-lymphocytes | |
dc.subject | Th22 cells | |
dc.subject | Th9 cells | |
dc.subject | cytokine | |
dc.subject | virulence factor | |
dc.subject | Aggregatibacter actinomycetemcomitans | |
dc.subject | biofilm | |
dc.subject | cell differentiation | |
dc.subject | helper cell | |
dc.subject | human | |
dc.subject | immunology | |
dc.subject | pathogenicity | |
dc.subject | phenotype | |
dc.subject | secretion (process) | |
dc.subject | serotype | |
dc.subject | Th17 cell | |
dc.subject | Aggregatibacter actinomycetemcomitans | |
dc.subject | Biofilms | |
dc.subject | Cell Differentiation | |
dc.subject | Cytokines | |
dc.subject | Humans | |
dc.subject | Phenotype | |
dc.subject | Serogroup | |
dc.subject | T-Lymphocytes, Helper-Inducer | |
dc.subject | Th17 Cells | |
dc.subject | Virulence Factors | |
dc.title | Differential human Th22-lymphocyte response triggered by Aggregatibacter actinomycetemcomitans serotypes | |
dc.type | Article |