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Potential probiotic yeasts isolated from the fish gut protect zebrafish (Danio rerio) from a Vibrio anguillarum challenge

dc.contributor.authorCaruffo M.
dc.contributor.authorNavarrete N.
dc.contributor.authorSalgado O.
dc.contributor.authorDíaz A.
dc.contributor.authorLópez P.
dc.contributor.authorGarcía K.
dc.contributor.authorFeijóo C.G.
dc.contributor.authorNavarrete P.
dc.date.accessioned2020-09-02T22:14:15Z
dc.date.available2020-09-02T22:14:15Z
dc.date.issued2015
dc.identifier10.3389/fmicb.2015.01093
dc.identifier.citation6, OCT, -
dc.identifier.issn1664302X
dc.identifier.urihttps://hdl.handle.net/20.500.12728/3935
dc.descriptionDue to the negative consequences associated with the use of antibiotics, researchers, and food producers have studied alternatives, such as probiotics, for the control of fish diseases. The probiotic properties of yeasts in aquaculture have been scarcely considered. The present study investigated the probiotic properties of local yeast strains for aquaculture application in the protection of bacterial diseases. Yeast strains (n = 15), previously isolated from the intestinal gut of healthy salmonids, yellowtail, and croaker, were evaluated for their protection of zebrafish larvae following a Vibrio anguillarum challenge. We developed an infection model on zebrafish larvae with V. anguillarum, observing rapid mortality (≥50%) 5 days post-immersion challenge. Infection of Tg(Lyz:DsRed)nz50 larvae with fluorescent-marked V. anguillarum showed the oro-intestinal as the natural route of infection concomitant with an inflammatory response of the larvae reflected by neutrophil migration outside the hematopoietic tissue. Thirteen of 15 strains increased the percentage of larvae survival after the V. anguillarum challenge, although no yeast showed in vitro anti-V. anguillarum activity. In a subset of yeasts, we explored yeast-larvae interactions using fluorescent yeast and evaluated larvae colonization by culture analysis. All fluorescent yeasts were located in the gastrointestinal tract until 5 days post-inoculation (dpi). Yeasts reached 103 CFU/larvae at 0 dpi, although the persistence until 5 dpi of the viable yeast in the gut was different among the strains. These results reveal that some yeasts isolated from the gut of fish could be potential probiotics, reducing the mortality associated to V. anguillarum challenge, and suggest that gut colonization could be involved in the protective effect. Future studies should elucidate other mechanisms involved in yeast protection and verify the beneficial effects of probiotic use in commercial fish species. © 2015 Caruffo, Navarrete, Salgado, Díaz, López, García, Feijóo and Navarrete.
dc.language.isoen
dc.publisherFrontiers Media S.A.
dc.subjectAquaculture
dc.subjectProbiotic
dc.subjectV. anguillarum
dc.subjectYeast
dc.subjectZebrafish model system
dc.subjectbiological product
dc.subjectprobiotic agent
dc.subjectArticle
dc.subjectbacterial growth
dc.subjectcell migration
dc.subjectcell viability
dc.subjectcolony forming unit
dc.subjecthost parasite interaction
dc.subjectinhibition zone
dc.subjectmicrobial colonization
dc.subjectneutrophil count
dc.subjectnonhuman
dc.subjectprotein expression
dc.subjectsurvival rate
dc.subjectVibrio anguillarum
dc.subjectzebra fish
dc.titlePotential probiotic yeasts isolated from the fish gut protect zebrafish (Danio rerio) from a Vibrio anguillarum challenge
dc.typeArticle


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