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Antifungal effect of polygodial on Botrytis cinerea, a fungal pathogen affecting table grapes

dc.contributor.authorCarrasco H.
dc.contributor.authorRobles-Kelly C.
dc.contributor.authorRubio J.
dc.contributor.authorOlea A.F.
dc.contributor.authorMartínez R.
dc.contributor.authorSilva-Moreno E.
dc.date.accessioned2020-09-02T22:14:14Z
dc.date.available2020-09-02T22:14:14Z
dc.date.issued2017
dc.identifier10.3390/ijms18112251
dc.identifier.citation18, 11, -
dc.identifier.issn16616596
dc.identifier.urihttps://hdl.handle.net/20.500.12728/3925
dc.descriptionThe antifungal activity of polygodial, a secondary metabolite extracted from Canelo, on mycelial growth of different Botrytis cinerea isolates has been evaluated. The results show that polygodial affects growth of normal and resistant isolates of B. cinerea with EC50 values ranging between 117 and 175 ppm. In addition, polygodial markedly decreases the germination of B. cinerea, i.e., after six hours of incubation the percentage of germination decreases from 92% (control) to 25% and 5% in the presence of 20 ppm and 80 ppm of polygodial, respectively. Morphological studies indicate that conidia treated with polygodial are smaller, with irregular membrane border, and a lot of cell debris, as compared to conidia in the control. The existence of polygodial-induced membrane damage was confirmed by SYTOX® Green uptake assay. Gene expression studies confirm that the effect of polygodial on B. cinerea is mainly attributed to inhibition of germination and appears at early stages of B. cinerea development. On the other hand, drimenol, a drimane with chemical structure quite similar to polygodial, inhibits the mycelial growth efficiently. Thus, both compounds inhibit mycelial growth by different mechanisms. The different antifungal activities of these compounds are discussed in terms of the electronic density on the double bond. © 2017 by the authors. Licensee MDPI, Basel, Switzerland.
dc.language.isoen
dc.publisherMDPI AG
dc.subjectAntifungal activity
dc.subjectBotrytis cinerea
dc.subjectPolygodial
dc.subjectantifungal agent
dc.subjectfungicide
dc.subjectiprodione
dc.subjectpolygodial
dc.subjectreactive oxygen metabolite
dc.subjectsytox
dc.subjectantifungal agent
dc.subjectfungicide
dc.subjectpolygodial
dc.subjectsesquiterpene
dc.subjectantifungal activity
dc.subjectArticle
dc.subjectBotrytis cinerea
dc.subjectcolumn chromatography
dc.subjectconidium
dc.subjectcontrolled study
dc.subjectEC50
dc.subjectextraction
dc.subjectfungus isolation
dc.subjectgene expression
dc.subjectgermination
dc.subjectgrape
dc.subjectinfectious agent
dc.subjectmicrophotography
dc.subjectmorphology
dc.subjectmycelial growth
dc.subjectnonhuman
dc.subjectpurification
dc.subjectBotrytis
dc.subjectchemistry
dc.subjectdrug effect
dc.subjectgene expression regulation
dc.subjectmicrobial sensitivity test
dc.subjectmicrobiology
dc.subjectVitis
dc.subjectAntifungal Agents
dc.subjectBotrytis
dc.subjectFungicides, Industrial
dc.subjectGene Expression Regulation, Fungal
dc.subjectMicrobial Sensitivity Tests
dc.subjectSesquiterpenes
dc.subjectVitis
dc.titleAntifungal effect of polygodial on Botrytis cinerea, a fungal pathogen affecting table grapes
dc.typeArticle


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