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Expression of hMLH1 and hMSH2 proteins in ameloblastomas and tooth germs

dc.contributor.authorBologna-Molina R.
dc.contributor.authorPereira-Prado V.
dc.contributor.authorSánchez-Romero C.
dc.contributor.authorTapia-Repetto G.
dc.contributor.authorSoria S.
dc.contributor.authorHernandez M.
dc.contributor.authorGónzalez-Gónzalez R.
dc.contributor.authorMolina-Frechero N.
dc.contributor.authorMikami T.
dc.date.accessioned2020-09-02T22:13:37Z
dc.date.available2020-09-02T22:13:37Z
dc.date.issued2018
dc.identifier10.4317/medoral.22210
dc.identifier.citation23, 2, e126-e131
dc.identifier.issn16984447
dc.identifier.urihttps://hdl.handle.net/20.500.12728/3815
dc.descriptionBackground: Mismatch repair proteins (MMRPs) are a group of nuclear enzymes that participate in the repair of base mismatches that occur during DNA replication in all proliferating cells. The most studied MMRPs are hMSH2 and hMLH1, which are known to be highly expressed in normal tissues. A loss of MMRPs leads to the accumulation of DNA replication errors in proliferating cells. Ki-67 is a biomarker regarded to be the gold-standard tool for determining cell proliferation by immunohistochemical methods. The aim of this study was to investigate the immunohistochemical expression of hMLH1, hMSH2 and Ki-67 proteins in ameloblastomas and tooth germs, to contribute to the understanding of the development of this odontogenic neoplasm. Material and Methods: Immunohistochemical assays to determine the presence of proteins hMSH2, hMLH1 and Ki-67 were performed in 80 ameloblastomas (40 solid and 40 unicystic) and five tooth germs. Results: Unicystic ameloblastomas showed higher MMRP expression (hMLH1: 62.5 ± 43.4; hMSH2: 83.3 ± 47.8) than did solid ameloblastomas (hMLH1: 59.4 ± 13.5; hMSH2: 75.8 ± 40.2). Additionally, the cell proliferation index assessed by Ki-67 was inversely proportional to the expression of MMRP. Comparison between tooth germs and ameloblastoma revealed significantly higher expression of hMLH1, hMSH2 and Ki-67 in tooth germs (p=0.02). Conclusions: The differences of MMRP and Ki-67 immunoexpression between ameloblastomas and tooth germ suggest that alterations in the MMRP mechanisms could participate in the biological behavior of ameloblastomas. © Medicina Oral S. L. C.I.F. B.
dc.language.isoen
dc.publisherMedicina Oral S.L.
dc.subjectAmeloblastomas
dc.subjectHMLH1
dc.subjectHMSH2
dc.subjectKi-67
dc.subjectTooth germs
dc.subjectDNA mismatch repair protein MSH2
dc.subjectKi 67 antigen
dc.subjectmismatch repair protein
dc.subjectMutL protein homolog 1
dc.subjectDNA mismatch repair protein MSH2
dc.subjectKi 67 antigen
dc.subjectMLH1 protein, human
dc.subjectMSH2 protein, human
dc.subjectMutL protein homolog 1
dc.subjectameloblastoma
dc.subjectArticle
dc.subjectcarcinogenesis
dc.subjectcell proliferation
dc.subjectcell proliferation assay
dc.subjectcontrolled study
dc.subjectdeacetylation
dc.subjectDNA methylation assay
dc.subjectDNA repair
dc.subjectDNA replication
dc.subjectgene expression
dc.subjectgene mutation
dc.subjectgenetic analysis
dc.subjecthistone acetylation
dc.subjecthuman
dc.subjecthuman cell
dc.subjecthuman tissue
dc.subjectimmunohistochemistry
dc.subjectmajor clinical study
dc.subjectnext generation sequencing
dc.subjectobservational study
dc.subjectodontogenic tumor
dc.subjectprotein expression
dc.subjecttooth germ
dc.subjectameloblastoma
dc.subjectbiosynthesis
dc.subjectjaw tumor
dc.subjectmetabolism
dc.subjecttooth germ
dc.subjectAmeloblastoma
dc.subjectHumans
dc.subjectImmunohistochemistry
dc.subjectJaw Neoplasms
dc.subjectKi-67 Antigen
dc.subjectMutL Protein Homolog 1
dc.subjectMutS Homolog 2 Protein
dc.subjectTooth Germ
dc.titleExpression of hMLH1 and hMSH2 proteins in ameloblastomas and tooth germs
dc.typeArticle


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