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Surfaces based on amino acid functionalized polyelectrolyte films towards active surfaces for enzyme immobilization

dc.contributor.authorBriones X.
dc.contributor.authorVillalobos V.
dc.contributor.authorQueneau Y.
dc.contributor.authorDanna C.S.
dc.contributor.authorMuñoz R.
dc.contributor.authorRíos H.E.
dc.contributor.authorPavez J.
dc.contributor.authorPáez M.
dc.contributor.authorCabrera R.
dc.contributor.authorTamayo L.
dc.contributor.authorUrzúa M.D.
dc.date.accessioned2020-09-02T22:13:33Z
dc.date.available2020-09-02T22:13:33Z
dc.date.issued2019
dc.identifier10.1016/j.msec.2019.109938
dc.identifier.citation104, , -
dc.identifier.issn09284931
dc.identifier.urihttps://hdl.handle.net/20.500.12728/3792
dc.descriptionSurface based on polyelectrolytes functionalized with amino acids onto amino-terminated solid surfaces of silicon wafers was prepared, with the purpose of evaluate the chemical functionality of the polyelectrolyte films in adsorption and catalytic activity of an enzyme. In this work, the adsorption of the enzyme glucose 6-phosphate dehydrogenase from Leuconostoc mesenteroides (LmG6PD) was studied as model. The polyelectrolytes were obtained from poly (maleic anhydride-alt-vinylpyrrolidone) [poly(MA-alt-VP)] and functionalized with amino acids of different hydropathy index: glutamine (Gln), tyrosine (Tyr) and methionine (Met). The polyelectrolytes were adsorbed onto the amino-terminated silicon wafer at pH 3.5 and 4.5 and at low and high ionic strength. At low ionic strength and pH 3.5, the largest quantity of adsorbed polyelectrolyte was on the films containing glutamine moiety as the most hydrophilic amino acid in the side chain of polymer chain (5.88 mg/m2), whereas at high ionic strength and pH 4.5, the lowest quantity was in films containing tyrosine moiety in the side chain (1.88 mg/m2). The films were characterized by ellipsometry, contact angle measurements and atomic force microscopy (AFM). The polyelectrolyte films showed a moderate degree of hydrophobicity, the methionine derivative being the most hydrophobic film. With the aim of evaluate the effect of the amino acid moieties on the ability of the surface to adsorb enzymes, we study the activity of the enzyme on these surfaces. We observed that the polarity of the side chain of the amino acid in the polyelectrolyte affected the quantity of LmG6PD adsorbed, as well as its specific activity, showing that films prepared from poly(MA-alt-VP) functionalized with Met provide the best enzymatic performance. The results obtained demonstrated that the surfaces prepared from polyelectrolytes functionalized with amino acids could be an attractive and simple platform for the immobilization of enzymes, which could be of interest for biocatalysis applications. © 2019 Elsevier B.V.
dc.language.isoen
dc.publisherElsevier Ltd
dc.subjectAmino acid functionalization
dc.subjectEnzyme adsorption
dc.subjectGlucose 6-phosphate dehydrogenase
dc.subjectPolyelectrolytes
dc.subjectSurfaces
dc.subjectAdsorption
dc.subjectAtomic force microscopy
dc.subjectCatalyst activity
dc.subjectContact angle
dc.subjectEnzyme activity
dc.subjectEnzyme immobilization
dc.subjectFilm preparation
dc.subjectGlucose
dc.subjectHydrophobicity
dc.subjectIonic strength
dc.subjectPolyelectrolytes
dc.subjectPolymer films
dc.subjectSilicon wafers
dc.subjectSurfaces
dc.subjectChemical functionality
dc.subjectEnzyme adsorption
dc.subjectFunctionalizations
dc.subjectHigh ionic strength
dc.subjectLeuconostoc mesenteroides
dc.subjectLow ionic strength
dc.subjectPolyelectrolyte films
dc.subjectSpecific activity
dc.subjectAmino acids
dc.subjectamino acid
dc.subjectglucose 6 phosphate dehydrogenase
dc.subjectimmobilized enzyme
dc.subjectnicotinamide adenine dinucleotide
dc.subjectpolyelectrolyte
dc.subjectadsorption
dc.subjectbiosynthesis
dc.subjectcarbon nuclear magnetic resonance
dc.subjectchemistry
dc.subjectenzymology
dc.subjectinfrared spectroscopy
dc.subjectLeuconostoc
dc.subjectmetabolism
dc.subjectwettability
dc.subjectAdsorption
dc.subjectAmino Acids
dc.subjectCarbon-13 Magnetic Resonance Spectroscopy
dc.subjectEnzymes, Immobilized
dc.subjectGlucosephosphate Dehydrogenase
dc.subjectLeuconostoc
dc.subjectNAD
dc.subjectPolyelectrolytes
dc.subjectSpectroscopy, Fourier Transform Infrared
dc.subjectWettability
dc.titleSurfaces based on amino acid functionalized polyelectrolyte films towards active surfaces for enzyme immobilization
dc.typeArticle


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