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Conditioned Medium of Human Adipose Mesenchymal Stem Cells Increases Wound Closure and Protects Human Astrocytes Following Scratch Assay In Vitro

dc.contributor.authorBaez-Jurado E.
dc.contributor.authorHidalgo-Lanussa O.
dc.contributor.authorGuio-Vega G.
dc.contributor.authorAshraf G.M.
dc.contributor.authorEcheverria V.
dc.contributor.authorAliev G.
dc.contributor.authorBarreto G.E.
dc.date.accessioned2020-09-02T22:12:36Z
dc.date.available2020-09-02T22:12:36Z
dc.date.issued2018
dc.identifier10.1007/s12035-017-0771-4
dc.identifier.citation55, 6, 5377-5392
dc.identifier.issn08937648
dc.identifier.urihttps://hdl.handle.net/20.500.12728/3668
dc.descriptionAstrocytes perform essential functions in the preservation of neural tissue. For this reason, these cells can respond with changes in gene expression, hypertrophy, and proliferation upon a traumatic brain injury event (TBI). Different therapeutic strategies may be focused on preserving astrocyte functions and favor a non-generalized and non-sustained protective response over time post-injury. A recent strategy has been the use of the conditioned medium of human adipose mesenchymal stem cells (CM-hMSCA) as a therapeutic strategy for the treatment of various neuropathologies. However, although there is a lot of information about its effect on neuronal protection, studies on astrocytes are scarce and its specific action in glial cells is not well explored. In the present study, the effects of CM-hMSCA on human astrocytes subjected to scratch assay were assessed. Our findings indicated that CM-hMSCA improved cell viability, reduced nuclear fragmentation, and preserved mitochondrial membrane potential. These effects were accompanied by morphological changes and an increased polarity index thus reflecting the ability of astrocytes to migrate to the wound stimulated by CM-hMSCA. In conclusion, CM-hMSCA may be considered as a promising therapeutic strategy for the protection of astrocyte function in brain pathologies. © 2017, Springer Science+Business Media, LLC.
dc.language.isoen
dc.publisherHumana Press Inc.
dc.subjectBrain injury
dc.subjectConditioned medium
dc.subjectHuman astrocytes
dc.subjectMesenchymal stem cells
dc.subjectMigration
dc.subjectScratch
dc.subjectglutathione peroxidase
dc.subjectmanganese superoxide dismutase
dc.subjectantioxidant
dc.subjectadipose derived stem cell
dc.subjectArticle
dc.subjectastrocyte
dc.subjectcell function
dc.subjectcell migration
dc.subjectcell polarity
dc.subjectcell viability
dc.subjecthuman
dc.subjecthuman cell
dc.subjectin vitro study
dc.subjectmesenchymal stem cell
dc.subjectmitochondrial membrane potential
dc.subjectneuropathology
dc.subjectneuroprotection
dc.subjectprotein expression
dc.subjectwound closure
dc.subjectwound healing assay
dc.subjectadipose tissue
dc.subjectastrocyte
dc.subjectbioassay
dc.subjectbiological model
dc.subjectcell motion
dc.subjectcell shape
dc.subjectcell survival
dc.subjectconditioned medium
dc.subjectcytology
dc.subjectDNA fragmentation
dc.subjectdrug effect
dc.subjectmesenchymal stem cell
dc.subjectmetabolism
dc.subjectneuroprotection
dc.subjectpathology
dc.subjectpharmacology
dc.subjectwound healing
dc.subjectAdipose Tissue
dc.subjectAntioxidants
dc.subjectAstrocytes
dc.subjectBiological Assay
dc.subjectCell Movement
dc.subjectCell Shape
dc.subjectCell Survival
dc.subjectCulture Media, Conditioned
dc.subjectDNA Fragmentation
dc.subjectHumans
dc.subjectMembrane Potential, Mitochondrial
dc.subjectMesenchymal Stem Cells
dc.subjectModels, Biological
dc.subjectNeuroprotection
dc.subjectWound Healing
dc.titleConditioned Medium of Human Adipose Mesenchymal Stem Cells Increases Wound Closure and Protects Human Astrocytes Following Scratch Assay In Vitro
dc.typeArticle


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