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Evidence for the facultative intracellular behaviour of the fish pathogen Vibrio ordalii

dc.contributor.authorAvendaño-Herrera R.
dc.contributor.authorArias-Muñoz E.
dc.contributor.authorRojas V.
dc.contributor.authorToranzo A.E.
dc.contributor.authorPoblete-Morales M.
dc.contributor.authorCórdova C.
dc.contributor.authorIrgang R.
dc.date.accessioned2020-09-02T22:12:33Z
dc.date.available2020-09-02T22:12:33Z
dc.date.issued2019
dc.identifier10.1111/jfd.13072
dc.identifier.citation42, 10, 1447-1455
dc.identifier.issn01407775
dc.identifier.urihttps://hdl.handle.net/20.500.12728/3649
dc.descriptionVibrio ordalii is an extracellular, Gram-negative bacterium that produces vibriosis in salmonids. While pathogenesis is not fully understood, this bacterium has numerous likely genes for adhesion, colonization, invasion factors and, as recently suggested, intracellular behaviour. Therefore, this study aimed to clarify possible intracellular behaviour for V. ordalii Vo-LM-18 and ATCC 33509T in the fish-cell lines SHK-1 and CHSE-214. Confocal microscopy revealed Vo-LM-18 and ATCC 33509T inside cytoplasm in both fish-cell lines at 4 hr post-inoculation (hpi). At 8 and 16 hpi, the proportion of fish cells invaded by both strains increased. Moreover, intracellular V. ordalii were observed after 8 hpi inside mouse embryonic fibroblasts (MEF), demonstrating that entry was not due to a cellular phagocytosis process. Flow cytometry confirmed immunocytochemistry results, with both V. ordalii evidencing statistically significant differences in the number of infected cells between 8 and 16 hpi. Interestingly, V. ordalii infection did not significantly damage fish cells, as determined by LDH liberation. Viable counts at 8 hpi detected, on average for both lines, 176 ± 47 CFU/ml of culturable intracellular Vo-LM-18 and ATCC 33509T cells. These in vitro findings support the facultative intracellular behaviour of V. ordalii and may be of importance for understanding pathogenicity and survival in aquatic environments. © 2019 John Wiley & Sons Ltd
dc.language.isoen
dc.publisherBlackwell Publishing Ltd
dc.subjectintracellular facultative
dc.subjectVibriosis
dc.subjectvirulence mechanism
dc.subjectanimal
dc.subjectcell line
dc.subjectconfocal microscopy
dc.subjectfish disease
dc.subjectflow cytometry
dc.subjectindirect fluorescent antibody technique
dc.subjectmicrobiology
dc.subjectphysiology
dc.subjectsalmonine
dc.subjectveterinary medicine
dc.subjectVibrio
dc.subjectvibriosis
dc.subjectAnimals
dc.subjectCell Line
dc.subjectFish Diseases
dc.subjectFlow Cytometry
dc.subjectFluorescent Antibody Technique, Indirect
dc.subjectMicroscopy, Confocal
dc.subjectSalmon
dc.subjectVibrio
dc.subjectVibrio Infections
dc.titleEvidence for the facultative intracellular behaviour of the fish pathogen Vibrio ordalii
dc.typeArticle


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