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Blockade of Neuroglobin Reduces Protection of Conditioned Medium from Human Mesenchymal Stem Cells in Human Astrocyte Model (T98G) Under a Scratch Assay

dc.contributor.authorBaez-Jurado E.
dc.contributor.authorVega G.G.
dc.contributor.authorAliev G.
dc.contributor.authorTarasov V.V.
dc.contributor.authorEsquinas P.
dc.contributor.authorEcheverria V.
dc.contributor.authorBarreto G.E.
dc.date.accessioned2020-09-02T22:12:21Z
dc.date.available2020-09-02T22:12:21Z
dc.date.issued2018
dc.identifier10.1007/s12035-017-0481-y
dc.identifier.citation55, 3, 2285-2300
dc.identifier.issn08937648
dc.identifier.urihttps://hdl.handle.net/20.500.12728/3572
dc.descriptionPrevious studies have indicated that paracrine factors (conditioned medium) increase wound closure and reduce reactive oxygen species in a traumatic brain injury in vitro model. Although the beneficial effects of conditioned medium from human adipose tissue-derived mesenchymal stem cells (hMSCA-CM) have been previously suggested for various neurological diseases, their actions on astrocytic cells are not well understood. In this study, we have explored the effect of hMSCA-CM on human astrocyte model (T98G cells) subjected to scratch assay. Our results indicated that hMSCA-CM improved cell viability, reduced nuclear fragmentation, attenuated the production of reactive oxygen species, and preserved mitochondrial membrane potential and ultrastructural parameters. In addition, hMSCA-CM upregulated neuroglobin in T98G cells and the genetic silencing of this protein prevented the protective action of hMSCA-CM on damaged cells, suggesting that neuroglobin is mediating, at least in part, the protective effect of hMSCA-CM. Overall, this evidence suggests that the use of hMSCA-CM is a promising therapeutic strategy for the protection of astrocytic cells in central nervous system (CNS) pathologies. © 2017, Springer Science+Business Media New York.
dc.language.isoen
dc.publisherHumana Press Inc.
dc.subjectAdipose tissue
dc.subjectAstrocytes
dc.subjectConditioned medium
dc.subjectMesenchymal stem cells
dc.subjectNeuroglobin
dc.subjectScratch assay
dc.subjectneuroglobin
dc.subjectreactive oxygen metabolite
dc.subjectneuroglobin
dc.subjectneuroprotective agent
dc.subjectNGB protein, human
dc.subjectArticle
dc.subjectastrocyte
dc.subjectcell nucleus
dc.subjectcell protection
dc.subjectcell ultrastructure
dc.subjectcell viability
dc.subjectcentral nervous system disease
dc.subjectcontrolled study
dc.subjectgene silencing
dc.subjecthuman
dc.subjecthuman cell
dc.subjectmesenchymal stem cell
dc.subjectmitochondrial membrane potential
dc.subjectT98G cell line
dc.subjectupregulation
dc.subjectwound healing assay
dc.subjectadult
dc.subjectantagonists and inhibitors
dc.subjectastrocyte
dc.subjectcell culture
dc.subjectcell line
dc.subjectconditioned medium
dc.subjectdrug effect
dc.subjectmesenchymal stem cell
dc.subjectoxidative stress
dc.subjectpharmacology
dc.subjectphysical stimulation
dc.subjectphysiology
dc.subjectyoung adult
dc.subjectAdult
dc.subjectAstrocytes
dc.subjectCell Line
dc.subjectCells, Cultured
dc.subjectCulture Media, Conditioned
dc.subjectHumans
dc.subjectMesenchymal Stem Cells
dc.subjectNeuroglobin
dc.subjectNeuroprotective Agents
dc.subjectOxidative Stress
dc.subjectPhysical Stimulation
dc.subjectYoung Adult
dc.titleBlockade of Neuroglobin Reduces Protection of Conditioned Medium from Human Mesenchymal Stem Cells in Human Astrocyte Model (T98G) Under a Scratch Assay
dc.typeArticle


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