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Characterisation of the gene family encoding acetoacetyl-CoA thiolase in Arabidopsis

dc.contributor.authorAhumada I.
dc.contributor.authorCairó A.
dc.contributor.authorHemmerlin A.
dc.contributor.authorGonzález V.
dc.contributor.authorPateraki I.
dc.contributor.authorBach T.J.
dc.contributor.authorRodríguez-Concepción M.
dc.contributor.authorCampos N.
dc.contributor.authorBoronat A.
dc.date.accessioned2020-09-02T22:11:02Z
dc.date.available2020-09-02T22:11:02Z
dc.date.issued2008
dc.identifier10.1071/FP08012
dc.identifier.citation35, 11, 1100-1111
dc.identifier.issn14454408
dc.identifier.urihttps://hdl.handle.net/20.500.12728/3495
dc.descriptionThiolases are ubiquitous enzymes involved in many essential biochemical processes. Biosynthetic thiolases, also known as acetoacetyl-CoA thiolases (AACT), catalyse a reversible Claisen-type condensation of two acetyl-CoA molecules to form acetoacetyl-CoA. Here, we report the characterisation of two genes from Arabidopsis thaliana L., ACT1 and ACT2, which encode two closely related AACT isoforms (AACT1 and AACT2, respectively). Transient expression of constructs encoding AACT1 and AACT2 fused to GFP revealed that the two proteins show a different subcellular localisation. While AACT1 is found in peroxisomes, AACT2 localises in the cytosol and the nucleus. The peroxisomal localisation of AACT1 depends on the presence of a C-terminal peroxisomal targeting sequence (PTS1) motif (Ser-Ala-Leu) not previously found in other organisms. ACT1 and ACT2 genes are also differentially expressed. Whereas ACT2 is expressed at relatively high level in all plant tissues, the expression of ACT1 is restricted to roots and inflorescences and its transcript is present at very low levels. The obtained results are in agreement with the involvement of AACT2 in catalysing the first step of the mevalonate pathway. The metabolic function of AACT1 is not clear at present, although its particular peroxisomal localisation might exclude a role in isoprenoid biosynthesis. © CSIRO 2008.
dc.language.isoen
dc.publisherCSIRO
dc.subjectAcetyl-CoA
dc.subjectIsoprenoid biosynthesis
dc.subjectMevalonate pathway
dc.subjectPeroxisome
dc.subjectBiochemistry
dc.subjectBiosynthesis
dc.subjectCytology
dc.subjectEncoding (symbols)
dc.subjectGene encoding
dc.subjectLipids
dc.subjectAcetyl-CoA
dc.subjectArabidopsis thaliana
dc.subjectIsoprenoid biosynthesis
dc.subjectMevalonate pathway
dc.subjectPeroxisomal targeting sequences
dc.subjectPeroxisome
dc.subjectSub-cellular localisation
dc.subjectTransient expression
dc.subjectSignal encoding
dc.subjectcatalysis
dc.subjectenzyme activity
dc.subjectgenetic analysis
dc.subjectherb
dc.subjectmetabolism
dc.subjectArabidopsis
dc.subjectArabidopsis thaliana
dc.titleCharacterisation of the gene family encoding acetoacetyl-CoA thiolase in Arabidopsis
dc.typeArticle


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