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Gallic acid loaded PEO-core/zein-shell nanofibers for chemopreventive action on gallbladder cancer cells

dc.contributor.authorAcevedo F.
dc.contributor.authorHermosilla J.
dc.contributor.authorSanhueza C.
dc.contributor.authorMora-Lagos B.
dc.contributor.authorFuentes I.
dc.contributor.authorRubilar M.
dc.contributor.authorConcheiro A.
dc.contributor.authorAlvarez-Lorenzo C.
dc.date.accessioned2020-09-02T22:10:59Z
dc.date.available2020-09-02T22:10:59Z
dc.date.issued2018
dc.identifier10.1016/j.ejps.2018.04.009
dc.identifier.citation119, , 49-61
dc.identifier.issn09280987
dc.identifier.urihttps://hdl.handle.net/20.500.12728/3473
dc.descriptionCoaxial electrospinning was used to develop gallic acid (GA) loaded poly(ethylene oxide)/zein nanofibers in order to improve its chemopreventive action on human gallbladder cancer cells. Using a Plackett-Burman design, the effects of poly(ethylene oxide) and zein concentration and applied voltage on the diameter and morphology index of nanofibers were investigated. Coaxial nanofibers were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC). GA loading efficiency as high as 77% was obtained under optimal process conditions. The coaxial nanofibers controlled GA release in acid and neutral pH medium. Cytotoxicity and reactive oxygen species (ROS) production on gallbladder cancer cell lines GB-d1 and NOZ in the presence of GA-nanofibers were assessed. GA-nanofibers triggered an increase in the cellular cytotoxicity compared with free GA on GB-d1 and NOZ cells. Statistically significant differences were found in ROS levels of GA-nanofibers compared with free GA on NOZ cells. Differently, ROS production on GB-d1 cell line was similar. Based on these results, the coaxial nanofibers obtained in this study under optimized operational conditions offer an alternative for the development of a GA release system with improved chemopreventive action on gallbladder cancer cells. © 2018 Elsevier B.V.
dc.language.isoen
dc.publisherElsevier B.V.
dc.subjectBiopolymers
dc.subjectCoaxial electrospinning
dc.subjectGallbladder cancer cells
dc.subjectPoly(ethylene oxide)
dc.subjectReactive oxygen species
dc.subjectZein
dc.subjectgallic acid
dc.subjectmacrogol
dc.subjectnanofiber
dc.subjectreactive oxygen metabolite
dc.subjectzein
dc.subjectantineoplastic agent
dc.subjectgallic acid
dc.subjectmacrogol
dc.subjectnanofiber
dc.subjectreactive oxygen metabolite
dc.subjectzein
dc.subjectArticle
dc.subjectcancer prevention
dc.subjectconductance
dc.subjectcytotoxicity
dc.subjectdielectric constant
dc.subjectdifferential scanning calorimetry
dc.subjectelectric potential
dc.subjectelectrospinning
dc.subjectgallbladder cancer
dc.subjecthuman
dc.subjecthuman cell
dc.subjectin vitro study
dc.subjectinfrared spectroscopy
dc.subjectpH
dc.subjectpriority journal
dc.subjectscanning electron microscopy
dc.subjectsurface tension
dc.subjectthermogravimetry
dc.subjecttransmission electron microscopy
dc.subjectcell survival
dc.subjectchemistry
dc.subjectchemoprophylaxis
dc.subjectdrug effect
dc.subjectdrug release
dc.subjectgallbladder tumor
dc.subjectmetabolism
dc.subjecttumor cell line
dc.subjectAnticarcinogenic Agents
dc.subjectAntineoplastic Agents
dc.subjectCell Line, Tumor
dc.subjectCell Survival
dc.subjectChemoprevention
dc.subjectDrug Liberation
dc.subjectGallbladder Neoplasms
dc.subjectGallic Acid
dc.subjectHumans
dc.subjectHydrogen-Ion Concentration
dc.subjectNanofibers
dc.subjectPolyethylene Glycols
dc.subjectReactive Oxygen Species
dc.subjectZein
dc.titleGallic acid loaded PEO-core/zein-shell nanofibers for chemopreventive action on gallbladder cancer cells
dc.typeArticle


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