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Epitranscriptomic regulation of HIV-1 full-length RNA packaging
dc.contributor.author | Pereira-Montecinos, Camila | |
dc.contributor.author | Toro-Ascuy, Daniela | |
dc.contributor.author | Ananias-Saez, Catarina | |
dc.contributor.author | Gaete-Argel, Aracelly | |
dc.contributor.author | Rojas-Fuentes, Cecilia | |
dc.contributor.author | Riquelme-Barrios, Sebastian | |
dc.contributor.author | Rojas-Araya, Barbara | |
dc.contributor.author | Garcia-de-Gracia, Francisco | |
dc.contributor.author | Aguilera-Cortes, Paulina | |
dc.contributor.author | Chnaiderman, Jonas | |
dc.contributor.author | Acevedo, Monica L. | |
dc.contributor.author | Valiente-Echeverria, Fernando | |
dc.contributor.author | Soto-Rifo, Ricardo | |
dc.date.accessioned | 2024-04-11T06:09:13Z | |
dc.date.available | 2024-04-11T06:09:13Z | |
dc.date.issued | 2022 | |
dc.identifier | 10.1093/nar/gkac062 | |
dc.identifier.issn | 3051048 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12728/11158 | |
dc.description.abstract | During retroviral replication, the full-length RNA serves both as mRNA and genomic RNA. However, the mechanisms by which the HIV-1 Gag protein selects the two RNA molecules that will be packaged into nascent virions remain poorly understood. Here, we demonstrate that deposition of N-6-methyladenosine (m(6)A) regulates full-length RNA packaging. While m(6)A deposition by METTL3/METTL14 onto the full-length RNA was associated with increased Gag synthesis and reduced packaging, FTO-mediated demethylation promoted the incorporation of the full-length RNA into viral particles. Interestingly, HIV-1 Gag associates with the RNA demethylase FTO in the nucleus and contributes to full-length RNA demethylation. We further identified two highly conserved adenosines within the 5 '-UTR that have a crucial functional role in m(6)A methylation and packaging of the full-length RNA. Together, our data propose a novel epitranscriptomic mechanism allowing the selection of the HIV-1 full-length RNA molecules that will be used as viral genomes. | es_ES |
dc.description.sponsorship | FONDECYT [1160176, 1190156, 1180798, 1211547, 11180621]; CONICYT | es_ES |
dc.language.iso | en | es_ES |
dc.publisher | OXFORD UNIV PRESS | es_ES |
dc.title | Epitranscriptomic regulation of HIV-1 full-length RNA packaging | es_ES |
dc.type | Article | es_ES |