Oligo-Carrageenan Kappa Increases Expression of Genes Encoding Proteins Involved in Photosynthesis, C, N, and S Assimilation, and Growth in Arabidopsis thaliana
Autor
Méndez, Tamara
Fuentes, Alejandra
Cofre, Diego
Moenne, Alejandra
Laporte, Daniel
Resumen
To analyze the effect of oligo-carrageenan (OC) kappa in the stimulation of growth in Arabidopsis thaliana, plants were sprayed on leaves with an aqueous solution of OC kappa at 1 mg mL−1, 5 times every 2 days and cultivated for 5 or 15 additional days. Plants treated with OC kappa showed an increase in rosette diameter, fresh and dry weight, and primary root length. Plants treated with OC kappa once and cultivated for 0 to 24 h after treatment were subjected to transcriptomic analyses to identify differentially expressed genes, mainly at 12 h after treatment. Transcripts encoding proteins involved in growth and development and photosynthesis were upregulated as well as enzymes involved in primary metabolism. In addition, plants treated with OC kappa once and cultivated for 0 to 96 h showed increased levels of transcripts encoding enzymes involved in C, N, and S assimilation at 6 and 12 h after treatment that remain increased until 96 h. Therefore, OC kappa increased the expression of genes encoding proteins involved in photosynthesis, C, N, and S assimilation, and growth in A. thaliana. © 2023 by the authors.
Colecciones
Ítems relacionados
Mostrando ítems relacionados por Título, autor o materia.
-
Article
Transcriptomic analyses reveal increased expression of dioxygenases, monooxygenases, and other metabolizing enzymes involved in anthracene degradation in the marine alga Ulva lactuca (2024)
González, Alberto; Osorio, Héctor; Romero, Stephanie; Méndez, Patricia; Sepúlveda, Muriel; Laporte, Daniel; Gutierrez-Cutiño, Marlen; Santander, Rocío; Castro-Nallar, Eduardo; Moenne, Alejandra (Frontiers Media S.A., 2022)To analyze the mechanisms involved in anthracene (ANT) degradation in the marine alga Ulva lactuca, total RNA was obtained from the alga cultivated without ANT and with 5 μM of ANT for 24 h, and transcriptomic analyses ... -
Article
Cadmium Accumulation Involves Synthesis of Glutathione and Phytochelatins, and Activation of CDPK, CaMK, CBLPK, and MAPK Signaling Pathways in Ulva compressa (2021)
González, Alberto; Laporte, Daniel; Moenne, Alejandra (Frontiers Media S.A., 2021-06-21)In order to analyze the effect of cadmium in Ulva compressa (Chlorophyta), the alga was cultivated with 10, 25, and 50 μM of cadmium for 7 days, and the level of intracellular cadmium was determined. Intracellular cadmium ... -
Article
Ulva compressa from copper-polluted sites exhibits intracellular copper accumulation, increased expression of metallothioneins and copper-containing nanoparticles in chloroplasts (2021)
Espinoza, Daniela; González, Alberto; Pizarro, Jaime A.; Segura, Rodrigo A.; Laporte, Daniel; Rodríguez Rojas, Fernanda; Sáez, Claudio A.; Moenne, Alejandra (MDPI, 2021-10-01)In order to analyze the mechanisms involved in copper accumulation in Ulva compressa, algae were collected at control sites of central and northern Chile, and at two copper-polluted sites of northern Chile. The level of ...