Study of Cannabis Oils Obtained from Three Varieties of C. sativa and by Two Different Extraction Methods: Phytochemical Characterization and Biological Activities
Autor
Pino, Sebastián
Espinoza, Luis
Jara-Gutiérrez, Carlos
Villena, Joan
Olea, Andrés F.
Díaz, Katy
Resumen
Currently, much effort is being placed into obtaining extracts and/or essential oils from Cannabis sativa L. for specific therapeutic purposes or pharmacological compositions. These potential applications depend mainly on the phytochemical composition of the oils, which in turn are determined by the type of C. sativa and the extraction method used to obtain the oils. In this work, we have evaluated the contents of secondary metabolites, delta-9-tetrahydrocannabinol (THC), and cannabidiol (CBD), in addition to the total phenolic, flavonoids, and anthraquinone content in oils obtained using solid–liquid extraction (SLE) and supercritical fluid extraction (SCF). Different varieties of C. sativa were chosen by using the ratio of THC to CBD concentrations. Additionally, antioxidant, antifungal and anticancer activities on different cancer cell lines were evaluated in vitro. The results indicate that oils extracted by SLE, with high contents of CBD, flavonoids, and phenolic compounds, exhibit a high antioxidant capacity and induce a high decrease in the cell viability of the tested breast cancer cell line (MCF-7). The observed biological activities are attributed to the entourage effect, in which CBD, phenols and flavonoids play a key role. Therefore, it is concluded that the right selection of C. sativa variety and the solvent for SLE extraction method could be used to obtain the optimal oil composition to develop a natural anticancer agent. © 2023 by the authors.
Colecciones
Ítems relacionados
Mostrando ítems relacionados por Título, autor o materia.
-
Article
Ethanolic Extract from Fruits of Pintoa chilensis, a Chilean Extremophile Plant. Assessment of Antioxidant Activity and In Vitro Cytotoxicity (2024)
Arrieche, Dioni; Olea, Andrés F.; Jara-Gutiérrez, Carlos; Villena, Joan; Pardo-Baeza, Javier; García-Davis, Sara; Viteri, Rafael; Taborga, Lautaro; Carrasco, Héctor (Multidisciplinary Digital Publishing Institute (MDPI), 2024)Pintoa chilensis is a shrub with yellow flowers that reach up to two meters high, endemic of the Atacama Region in Chile. This species grows under special environmental conditions such as low altitude, arid areas, and ... -
Article
Antiproliferative effect and apoptotic activity of linear geranylphenol derivatives from phloroglucinol and orcinol (2020)
Taborga L.; Espinoza L.; Moller A.; Carrasco H.; Cuellar M.; Villena J. (Elsevier Ireland Ltd, 2016) -
Article
Cytotoxic and apoptotic effects of leptocarpin, a plant-derived sesquiterpene lactone, on human cancer cell lines (2020)
Bosio C.; Tomasoni G.; Martínez R.; Olea A.F.; Carrasco H.; Villena J. (Elsevier Ireland Ltd, 2015)