Use of Chitosan from Southern King Crab to Develop Films Functionalized with RGD Peptides for Potential Tissue Engineering Applications
Autor
Forero, Juan Carlos
Carvajal, Karina
Guzmán, Fanny
Acevedo, Cristian
Osses, Nelson
Santana, Paula
Resumen
Southern King Crab (SKC) represents an important fishery resource that has the potential to be a natural source of chitosan (CS) production. In tissue engineering, CS is very useful to generate biomaterials. However, CS has a lack of signaling molecules that facilitate cell–substrate interaction. Therefore, RGD (arginine–glycine–aspartic acid) peptides corresponding to the main integrin recognition site in extracellular matrix proteins have been used to improve the CS surface. The aim of this study was to evaluate in vitro cell adhesion and proliferation of CS films synthesized from SKC shell wastes functionalized with RGD peptides. The FTIR spectrum of CS isolated from SKC shells (SKC-CS) was comparable to commercial CS. Thermal properties of films showed similar endothermic peaks at 53.4 and 53.0 °C in commercial CS and SKC-CS, respectively. The purification and molecular masses of the synthesized RGD peptides were confirmed using HPLC and ESI-MS mass spectrometry, respectively. Mouse embryonic fibroblast cells showed higher adhesion on SKC-CS (1% w/v) film when it was functionalized with linear RGD peptides. In contrast, a cyclic RGD peptide showed similar adhesion to control peptide (RDG), but the highest cell proliferation was after 48 h of culture. This study shows that functionalization of SKC-CS films with linear or cyclic RGD peptides are useful to improve effects on cell adhesion or cell proliferation. Furthermore, our work contributes to knowledge of a new source of CS to synthesize constructs for tissue engineering applications. © 2023 by the authors.
Colecciones
Ítems relacionados
Mostrando ítems relacionados por Título, autor o materia.
-
Article
Immunodetection of rainbow trout IL-8 cleaved-peptide: Tissue bioavailability and potential antibacterial activity in a bacterial infection context (2021)
Sáenz-Martínez, Daniel E.; Santana, Paula Andrea; Aróstica, Mónica; Forero, Juan Carlos; Guzmán, Fanny; Mercado, Luis (Elsevier Ltd, 2021-11)Chemokines such as IL-8 are part of an important group of proinflammatory response molecules, as well as cell recruitment. However, it has been described in both higher vertebrates and fish that IL-8 has an additional ... -
Article
Stability of ACE2 Peptide Mimetics and Their Implications on the Application for SARS-CoV2 Detection (2024)
Santana, Paula A.; Álvarez, Claudio A.; Valenzuela, Santiago; Manchego, Alberto; Guzmán, Fanny; Tirapegui, Cristian; Ahumada, Manuel (MDPI, 2023)The SARS-CoV-2 worldwide outbreak prompted the development of several tools to detect and treat the disease. Among the new detection proposals, the use of peptides mimetics has surged as an alternative to avoid the use of ... -
Article
Effects of elderflower extract enriched with polyphenols on antioxidant defense of salmon leukocytes (2021)
Santana, Paula Andrea; Jara-Gutiérrez, Carlos; Mellado, Marco; Forero, Juan Carlos; Guzmán, Fanny; Barriga González, Andrés; Alberício, Fernando; Álvarez, Claudio Andrés (Pontificia Universidad Catolica de Valparaiso, 2021-07)Background: In fish farming, the plant extracts containing antioxidant compounds have been added to the diet for enhancing pathogen resistance. In vitro studies evaluating the antioxidant effect of herbal extracts on fish ...