Mostrar el registro sencillo del ítem

dc.contributor.authorGarcía-Mesa, Yolanda
dc.contributor.authorCuendias, Patricia
dc.contributor.authorAlonso-Guervós, Marta
dc.contributor.authorGarcía-Piqueras, Jorge
dc.contributor.authorMartín-Biedma, Benjamín
dc.contributor.authorCobo, Teresa
dc.contributor.authorGarcía-Suárez, Olivia
dc.contributor.authorVega, José A.
dc.date.accessioned2024-04-09T22:54:19Z
dc.date.available2024-04-09T22:54:19Z
dc.date.issued2024
dc.identifier10.1016/j.aanat.2023.152200
dc.identifier.issn09409602
dc.identifier.urihttps://hdl.handle.net/20.500.12728/10322
dc.description.abstractBackground: The cutaneous end organ complexes or cutaneous sensory corpuscles are specialized sensory organs associated to low-threshold mechanoreceptors. Mechano-gated proteins forming a part of ion channels have been detected in both the axon and terminal glial cells of Meissner corpuscles, a specific cutaneous end organ complex in the human glabrous skin. The main candidates to mechanotransduction in Meissner corpuscles are members of the Piezo family of cationic ion channels. PIEZO2 has been detected in the axon of these sensory structures whereas no data exists about the occurrence and cell localization of PIEZO1. Methods: Skin samples (n = 18) from the palmar aspect of the distal phalanx of the first and second fingers were analysed (8 female and 10 males; age range 26 to 61 26–61 years). Double immunofluorescence for PIEZO1 and PIEZO2 together with axonal or terminal glial cell markers was captured by laser confocal microscopy, and the percentage of PIEZOs positive Meissner corpuscles was evaluated. Results: MCs from human fingers showed variable morphology and degree of lobulation. Regarding the basic immunohistochemical profile, in all cases the axons were immunoreactive for neurofilament proteins, neuron specific enolase and synaptophysin, while the lamellar cells displayed strong S100P immunoreactivity. PIEZO1 was detected co-localizing with axonal markers, but never with terminal glial cell markers, in the 56% of Meissner corpuscles; weak but specific immunofluorescence was additionally detected in the epidermis, especially in basal keratinocytes. Similarly, PIEZO2 immunoreactivity was found restricted to the axon in the 85% of Meissner corpuscles. PIEZO2 positive Merkel cells were also regularly found. Conclusions: PIEZO1 and PIEZO2 are expressed exclusively in the axon of a subpopulation of human digital Meissner corpuscles, thus suggesting that not only PIEZO2, but also PIEZO1 may be involved in the mechanotransduction from low-threshold mechanoreceptors. © 2023 The Authorses_ES
dc.description.sponsorshipGobierno del Principado de Asturias, GPAes_ES
dc.language.isoenes_ES
dc.publisherElsevier GmbHes_ES
dc.subjectCutaneous end organ complexeses_ES
dc.subjectGlabrous skines_ES
dc.subjectMechanoproteinses_ES
dc.subjectMeissner corpuscleses_ES
dc.titleImmunohistochemical detection of PIEZO1 and PIEZO2 in human digital Meissner´s corpuscleses_ES
dc.typeArticlees_ES


Ficheros en el ítem

Thumbnail

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem